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cd73 membrane translocationmgat1 nature communications
Cd73 Membrane Translocationmgat1 Nature Communications, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 11 article reviews
Cd73 Membrane Translocationmgat1 Nature Communications, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd73+membrane+translocationmgat1+nature+communications/5%E2%80%B2-Nucleotidase/pm40229283-223-515-530
Average 94 stars, based on 11 article reviews
cd73 membrane translocationmgat1 nature communications - by Bioz Stars,
2026-09
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In Vitro:Article Title: MGAT1-Guided complex N-Glycans on CD73 regulate immune evasion in triple-negative breast cancer. Article Snippet: We initially developed an in vitro high-throughput screening assay to monitor the effect on MGAT1 enzymatic activity of a library of putative inhibitors (Fig. 6a). dc e g h j l m N401 N56 N311 N333 Open (inactive) CD73 dimer Closed (active) CD73 dimer Actin MGAT1-OE MGAT1-KD CD73 Dimer CD73 Monomer MDA-MD468 WT Glutaraldehyde - + - + - + 150 kDa 75 kDa 50 kDa MDA-MB468 MGAT1-OE MGAT1-KD SSC-B-H M em br an e C D 73 14.8% 27.3% 2.69% 5 mCD73 Membrane Dye MDA-MB468 MGAT1-OE MGAT1-KD 10 m a CD73 Split-GFP Scheme GFP 1-10 Linker 25aa WT/4NQ/480-537 truncation CD73 GFP 11x7 Linker 25aa WT/4NQ/480-537 truncation CD73 WT CD73 4NQ-CD73 Dimer-deficient CD73 40 x W at er Z oo m 2 .3 CD73 Dimer 00.100.1- 0.00 AD A TG FB 3 C XC L1 4 TG FB R 3 TG FB 2 TG FB R 1 C XC L1 2 TG FB 1 TH BS 3 C XC L9 TH BS 4 C XC L1 6 C XC L1 0 AD A2 EN TP D 1 N T5 E TH BS 2 TH BS 1 M G AT 1 id MGAT1 THBS1 THBS2 NT5E ENTPD1 ADA2 CXCL10 CXCL16 THBS4 CXCL9 THBS3 TGFB1 CXCL12 TGFBR1 TGFB2 TGFBR3 CXCL14 TGFB3 ADA id MDA-MB231 MGAT1 CD73 Actin WT 468 Control vector MGAT1 -OE MGAT1 -OE MGAT1 -KD1 MGAT1 -KD2 75 kDa 50 kDa MDA-MB468 MGAT1 CD73 Actin WT 468 Control vector MGAT1 -OE MGAT1 -OE MGAT1 -KD1 MGAT1 -KD2 75 kDa 50 kDa 50 kDa 42 kDa 50 kDa 42 kDa MDA-MB468 CD73 Actin Co ntr ol En do H PN Ga se F WT Co ntr ol En do H PN Ga se F MGAT1-OE Co ntr ol En do H PN Ga se F MGAT1-KD 75 kDa 50 kDa 42 kDa 42 kDa Actin MGAT1 -OE MGAT1 -KD CD73 Dimer CD73 Monomer MDA-MD468 WT 146 kDa 66 kDa 42 kDa 10 m k b f i Co ntr ol 23 1 VA MP 3-K D- g1 VA MP 3-K D- g2 0 20 40 60 80 Su rfa ce C D 73 + ce lls (% ) p < 0.0001 p < 0.0001 Co ntr ol 46 8 VA MP 3-K D- g1 VA MP 3-K D- g2 0 20 40 60 80 Su rfa ce C D 73 + ce lls (% ) p < 0.0001 p < 0.0001 MD A- MB 46 8 + 1 g/m l T HB S1 0.0 0.5 1.0 1.5 2.0 Ad en os in e co nc en tra tio n ( M ) p = 0.0007 MD A- MB 23 1 + 1 g/m l T HB S1 0 1 2 3 4 Ad en os in e co nc en tra tio n ( M ) p = 0.0009 CD73 CD73 MGAT1 Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly VAMP3 G ly Gly G ly G ly G ly G ly G ly G ly Gly G ly G ly G ly Gly T cells AMP Adenosine X Transport vesicles VAMP3 Failure on CD73 membrane translocationMGAT1 Nature Communications | (2025) 16:3552 10 An High Throughput Screening Assay:Article Title: MGAT1-Guided complex N-Glycans on CD73 regulate immune evasion in triple-negative breast cancer. Article Snippet: We initially developed an in vitro high-throughput screening assay to monitor the effect on MGAT1 enzymatic activity of a library of putative inhibitors (Fig. 6a). dc e g h j l m N401 N56 N311 N333 Open (inactive) CD73 dimer Closed (active) CD73 dimer Actin MGAT1-OE MGAT1-KD CD73 Dimer CD73 Monomer MDA-MD468 WT Glutaraldehyde - + - + - + 150 kDa 75 kDa 50 kDa MDA-MB468 MGAT1-OE MGAT1-KD SSC-B-H M em br an e C D 73 14.8% 27.3% 2.69% 5 mCD73 Membrane Dye MDA-MB468 MGAT1-OE MGAT1-KD 10 m a CD73 Split-GFP Scheme GFP 1-10 Linker 25aa WT/4NQ/480-537 truncation CD73 GFP 11x7 Linker 25aa WT/4NQ/480-537 truncation CD73 WT CD73 4NQ-CD73 Dimer-deficient CD73 40 x W at er Z oo m 2 .3 CD73 Dimer 00.100.1- 0.00 AD A TG FB 3 C XC L1 4 TG FB R 3 TG FB 2 TG FB R 1 C XC L1 2 TG FB 1 TH BS 3 C XC L9 TH BS 4 C XC L1 6 C XC L1 0 AD A2 EN TP D 1 N T5 E TH BS 2 TH BS 1 M G AT 1 id MGAT1 THBS1 THBS2 NT5E ENTPD1 ADA2 CXCL10 CXCL16 THBS4 CXCL9 THBS3 TGFB1 CXCL12 TGFBR1 TGFB2 TGFBR3 CXCL14 TGFB3 ADA id MDA-MB231 MGAT1 CD73 Actin WT 468 Control vector MGAT1 -OE MGAT1 -OE MGAT1 -KD1 MGAT1 -KD2 75 kDa 50 kDa MDA-MB468 MGAT1 CD73 Actin WT 468 Control vector MGAT1 -OE MGAT1 -OE MGAT1 -KD1 MGAT1 -KD2 75 kDa 50 kDa 50 kDa 42 kDa 50 kDa 42 kDa MDA-MB468 CD73 Actin Co ntr ol En do H PN Ga se F WT Co ntr ol En do H PN Ga se F MGAT1-OE Co ntr ol En do H PN Ga se F MGAT1-KD 75 kDa 50 kDa 42 kDa 42 kDa Actin MGAT1 -OE MGAT1 -KD CD73 Dimer CD73 Monomer MDA-MD468 WT 146 kDa 66 kDa 42 kDa 10 m k b f i Co ntr ol 23 1 VA MP 3-K D- g1 VA MP 3-K D- g2 0 20 40 60 80 Su rfa ce C D 73 + ce lls (% ) p < 0.0001 p < 0.0001 Co ntr ol 46 8 VA MP 3-K D- g1 VA MP 3-K D- g2 0 20 40 60 80 Su rfa ce C D 73 + ce lls (% ) p < 0.0001 p < 0.0001 MD A- MB 46 8 + 1 g/m l T HB S1 0.0 0.5 1.0 1.5 2.0 Ad en os in e co nc en tra tio n ( M ) p = 0.0007 MD A- MB 23 1 + 1 g/m l T HB S1 0 1 2 3 4 Ad en os in e co nc en tra tio n ( M ) p = 0.0009 CD73 CD73 MGAT1 Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly VAMP3 G ly Gly G ly G ly G ly G ly G ly G ly Gly G ly G ly G ly Gly T cells AMP Adenosine X Transport vesicles VAMP3 Failure on CD73 membrane translocationMGAT1 Nature Communications | (2025) 16:3552 10 An Activity Assay:Article Title: MGAT1-Guided complex N-Glycans on CD73 regulate immune evasion in triple-negative breast cancer. Article Snippet: We initially developed an in vitro high-throughput screening assay to monitor the effect on MGAT1 enzymatic activity of a library of putative inhibitors (Fig. 6a). dc e g h j l m N401 N56 N311 N333 Open (inactive) CD73 dimer Closed (active) CD73 dimer Actin MGAT1-OE MGAT1-KD CD73 Dimer CD73 Monomer MDA-MD468 WT Glutaraldehyde - + - + - + 150 kDa 75 kDa 50 kDa MDA-MB468 MGAT1-OE MGAT1-KD SSC-B-H M em br an e C D 73 14.8% 27.3% 2.69% 5 mCD73 Membrane Dye MDA-MB468 MGAT1-OE MGAT1-KD 10 m a CD73 Split-GFP Scheme GFP 1-10 Linker 25aa WT/4NQ/480-537 truncation CD73 GFP 11x7 Linker 25aa WT/4NQ/480-537 truncation CD73 WT CD73 4NQ-CD73 Dimer-deficient CD73 40 x W at er Z oo m 2 .3 CD73 Dimer 00.100.1- 0.00 AD A TG FB 3 C XC L1 4 TG FB R 3 TG FB 2 TG FB R 1 C XC L1 2 TG FB 1 TH BS 3 C XC L9 TH BS 4 C XC L1 6 C XC L1 0 AD A2 EN TP D 1 N T5 E TH BS 2 TH BS 1 M G AT 1 id MGAT1 THBS1 THBS2 NT5E ENTPD1 ADA2 CXCL10 CXCL16 THBS4 CXCL9 THBS3 TGFB1 CXCL12 TGFBR1 TGFB2 TGFBR3 CXCL14 TGFB3 ADA id MDA-MB231 MGAT1 CD73 Actin WT 468 Control vector MGAT1 -OE MGAT1 -OE MGAT1 -KD1 MGAT1 -KD2 75 kDa 50 kDa MDA-MB468 MGAT1 CD73 Actin WT 468 Control vector MGAT1 -OE MGAT1 -OE MGAT1 -KD1 MGAT1 -KD2 75 kDa 50 kDa 50 kDa 42 kDa 50 kDa 42 kDa MDA-MB468 CD73 Actin Co ntr ol En do H PN Ga se F WT Co ntr ol En do H PN Ga se F MGAT1-OE Co ntr ol En do H PN Ga se F MGAT1-KD 75 kDa 50 kDa 42 kDa 42 kDa Actin MGAT1 -OE MGAT1 -KD CD73 Dimer CD73 Monomer MDA-MD468 WT 146 kDa 66 kDa 42 kDa 10 m k b f i Co ntr ol 23 1 VA MP 3-K D- g1 VA MP 3-K D- g2 0 20 40 60 80 Su rfa ce C D 73 + ce lls (% ) p < 0.0001 p < 0.0001 Co ntr ol 46 8 VA MP 3-K D- g1 VA MP 3-K D- g2 0 20 40 60 80 Su rfa ce C D 73 + ce lls (% ) p < 0.0001 p < 0.0001 MD A- MB 46 8 + 1 g/m l T HB S1 0.0 0.5 1.0 1.5 2.0 Ad en os in e co nc en tra tio n ( M ) p = 0.0007 MD A- MB 23 1 + 1 g/m l T HB S1 0 1 2 3 4 Ad en os in e co nc en tra tio n ( M ) p = 0.0009 CD73 CD73 MGAT1 Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly VAMP3 G ly Gly G ly G ly G ly G ly G ly G ly Gly G ly G ly G ly Gly T cells AMP Adenosine X Transport vesicles VAMP3 Failure on CD73 membrane translocationMGAT1 Nature Communications | (2025) 16:3552 10 An Multiple Displacement Amplification:Article Title: MGAT1-Guided complex N-Glycans on CD73 regulate immune evasion in triple-negative breast cancer. Article Snippet: We initially developed an in vitro high-throughput screening assay to monitor the effect on MGAT1 enzymatic activity of a library of putative inhibitors (Fig. 6a). dc e g h j l m N401 N56 N311 N333 Open (inactive) CD73 dimer Closed (active) CD73 dimer Actin MGAT1-OE MGAT1-KD CD73 Dimer CD73 Monomer MDA-MD468 WT Glutaraldehyde - + - + - + 150 kDa 75 kDa 50 kDa MDA-MB468 MGAT1-OE MGAT1-KD SSC-B-H M em br an e C D 73 14.8% 27.3% 2.69% 5 mCD73 Membrane Dye MDA-MB468 MGAT1-OE MGAT1-KD 10 m a CD73 Split-GFP Scheme GFP 1-10 Linker 25aa WT/4NQ/480-537 truncation CD73 GFP 11x7 Linker 25aa WT/4NQ/480-537 truncation CD73 WT CD73 4NQ-CD73 Dimer-deficient CD73 40 x W at er Z oo m 2 .3 CD73 Dimer 00.100.1- 0.00 AD A TG FB 3 C XC L1 4 TG FB R 3 TG FB 2 TG FB R 1 C XC L1 2 TG FB 1 TH BS 3 C XC L9 TH BS 4 C XC L1 6 C XC L1 0 AD A2 EN TP D 1 N T5 E TH BS 2 TH BS 1 M G AT 1 id MGAT1 THBS1 THBS2 NT5E ENTPD1 ADA2 CXCL10 CXCL16 THBS4 CXCL9 THBS3 TGFB1 CXCL12 TGFBR1 TGFB2 TGFBR3 CXCL14 TGFB3 ADA id MDA-MB231 MGAT1 CD73 Actin WT 468 Control vector MGAT1 -OE MGAT1 -OE MGAT1 -KD1 MGAT1 -KD2 75 kDa 50 kDa MDA-MB468 MGAT1 CD73 Actin WT 468 Control vector MGAT1 -OE MGAT1 -OE MGAT1 -KD1 MGAT1 -KD2 75 kDa 50 kDa 50 kDa 42 kDa 50 kDa 42 kDa MDA-MB468 CD73 Actin Co ntr ol En do H PN Ga se F WT Co ntr ol En do H PN Ga se F MGAT1-OE Co ntr ol En do H PN Ga se F MGAT1-KD 75 kDa 50 kDa 42 kDa 42 kDa Actin MGAT1 -OE MGAT1 -KD CD73 Dimer CD73 Monomer MDA-MD468 WT 146 kDa 66 kDa 42 kDa 10 m k b f i Co ntr ol 23 1 VA MP 3-K D- g1 VA MP 3-K D- g2 0 20 40 60 80 Su rfa ce C D 73 + ce lls (% ) p < 0.0001 p < 0.0001 Co ntr ol 46 8 VA MP 3-K D- g1 VA MP 3-K D- g2 0 20 40 60 80 Su rfa ce C D 73 + ce lls (% ) p < 0.0001 p < 0.0001 MD A- MB 46 8 + 1 g/m l T HB S1 0.0 0.5 1.0 1.5 2.0 Ad en os in e co nc en tra tio n ( M ) p = 0.0007 MD A- MB 23 1 + 1 g/m l T HB S1 0 1 2 3 4 Ad en os in e co nc en tra tio n ( M ) p = 0.0009 CD73 CD73 MGAT1 Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly VAMP3 G ly Gly G ly G ly G ly G ly G ly G ly Gly G ly G ly G ly Gly T cells AMP Adenosine X Transport vesicles VAMP3 Failure on CD73 membrane translocationMGAT1 Nature Communications | (2025) 16:3552 10 An Membrane:Article Title: MGAT1-Guided complex N-Glycans on CD73 regulate immune evasion in triple-negative breast cancer. Article Snippet: We initially developed an in vitro high-throughput screening assay to monitor the effect on MGAT1 enzymatic activity of a library of putative inhibitors (Fig. 6a). dc e g h j l m N401 N56 N311 N333 Open (inactive) CD73 dimer Closed (active) CD73 dimer Actin MGAT1-OE MGAT1-KD CD73 Dimer CD73 Monomer MDA-MD468 WT Glutaraldehyde - + - + - + 150 kDa 75 kDa 50 kDa MDA-MB468 MGAT1-OE MGAT1-KD SSC-B-H M em br an e C D 73 14.8% 27.3% 2.69% 5 mCD73 Membrane Dye MDA-MB468 MGAT1-OE MGAT1-KD 10 m a CD73 Split-GFP Scheme GFP 1-10 Linker 25aa WT/4NQ/480-537 truncation CD73 GFP 11x7 Linker 25aa WT/4NQ/480-537 truncation CD73 WT CD73 4NQ-CD73 Dimer-deficient CD73 40 x W at er Z oo m 2 .3 CD73 Dimer 00.100.1- 0.00 AD A TG FB 3 C XC L1 4 TG FB R 3 TG FB 2 TG FB R 1 C XC L1 2 TG FB 1 TH BS 3 C XC L9 TH BS 4 C XC L1 6 C XC L1 0 AD A2 EN TP D 1 N T5 E TH BS 2 TH BS 1 M G AT 1 id MGAT1 THBS1 THBS2 NT5E ENTPD1 ADA2 CXCL10 CXCL16 THBS4 CXCL9 THBS3 TGFB1 CXCL12 TGFBR1 TGFB2 TGFBR3 CXCL14 TGFB3 ADA id MDA-MB231 MGAT1 CD73 Actin WT 468 Control vector MGAT1 -OE MGAT1 -OE MGAT1 -KD1 MGAT1 -KD2 75 kDa 50 kDa MDA-MB468 MGAT1 CD73 Actin WT 468 Control vector MGAT1 -OE MGAT1 -OE MGAT1 -KD1 MGAT1 -KD2 75 kDa 50 kDa 50 kDa 42 kDa 50 kDa 42 kDa MDA-MB468 CD73 Actin Co ntr ol En do H PN Ga se F WT Co ntr ol En do H PN Ga se F MGAT1-OE Co ntr ol En do H PN Ga se F MGAT1-KD 75 kDa 50 kDa 42 kDa 42 kDa Actin MGAT1 -OE MGAT1 -KD CD73 Dimer CD73 Monomer MDA-MD468 WT 146 kDa 66 kDa 42 kDa 10 m k b f i Co ntr ol 23 1 VA MP 3-K D- g1 VA MP 3-K D- g2 0 20 40 60 80 Su rfa ce C D 73 + ce lls (% ) p < 0.0001 p < 0.0001 Co ntr ol 46 8 VA MP 3-K D- g1 VA MP 3-K D- g2 0 20 40 60 80 Su rfa ce C D 73 + ce lls (% ) p < 0.0001 p < 0.0001 MD A- MB 46 8 + 1 g/m l T HB S1 0.0 0.5 1.0 1.5 2.0 Ad en os in e co nc en tra tio n ( M ) p = 0.0007 MD A- MB 23 1 + 1 g/m l T HB S1 0 1 2 3 4 Ad en os in e co nc en tra tio n ( M ) p = 0.0009 CD73 CD73 MGAT1 Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly VAMP3 G ly Gly G ly G ly G ly G ly G ly G ly Gly G ly G ly G ly Gly T cells AMP Adenosine X Transport vesicles VAMP3 Failure on CD73 membrane translocationMGAT1 Nature Communications | (2025) 16:3552 10 An Control:Article Title: MGAT1-Guided complex N-Glycans on CD73 regulate immune evasion in triple-negative breast cancer. Article Snippet: We initially developed an in vitro high-throughput screening assay to monitor the effect on MGAT1 enzymatic activity of a library of putative inhibitors (Fig. 6a). dc e g h j l m N401 N56 N311 N333 Open (inactive) CD73 dimer Closed (active) CD73 dimer Actin MGAT1-OE MGAT1-KD CD73 Dimer CD73 Monomer MDA-MD468 WT Glutaraldehyde - + - + - + 150 kDa 75 kDa 50 kDa MDA-MB468 MGAT1-OE MGAT1-KD SSC-B-H M em br an e C D 73 14.8% 27.3% 2.69% 5 mCD73 Membrane Dye MDA-MB468 MGAT1-OE MGAT1-KD 10 m a CD73 Split-GFP Scheme GFP 1-10 Linker 25aa WT/4NQ/480-537 truncation CD73 GFP 11x7 Linker 25aa WT/4NQ/480-537 truncation CD73 WT CD73 4NQ-CD73 Dimer-deficient CD73 40 x W at er Z oo m 2 .3 CD73 Dimer 00.100.1- 0.00 AD A TG FB 3 C XC L1 4 TG FB R 3 TG FB 2 TG FB R 1 C XC L1 2 TG FB 1 TH BS 3 C XC L9 TH BS 4 C XC L1 6 C XC L1 0 AD A2 EN TP D 1 N T5 E TH BS 2 TH BS 1 M G AT 1 id MGAT1 THBS1 THBS2 NT5E ENTPD1 ADA2 CXCL10 CXCL16 THBS4 CXCL9 THBS3 TGFB1 CXCL12 TGFBR1 TGFB2 TGFBR3 CXCL14 TGFB3 ADA id MDA-MB231 MGAT1 CD73 Actin WT 468 Control vector MGAT1 -OE MGAT1 -OE MGAT1 -KD1 MGAT1 -KD2 75 kDa 50 kDa MDA-MB468 MGAT1 CD73 Actin WT 468 Control vector MGAT1 -OE MGAT1 -OE MGAT1 -KD1 MGAT1 -KD2 75 kDa 50 kDa 50 kDa 42 kDa 50 kDa 42 kDa MDA-MB468 CD73 Actin Co ntr ol En do H PN Ga se F WT Co ntr ol En do H PN Ga se F MGAT1-OE Co ntr ol En do H PN Ga se F MGAT1-KD 75 kDa 50 kDa 42 kDa 42 kDa Actin MGAT1 -OE MGAT1 -KD CD73 Dimer CD73 Monomer MDA-MD468 WT 146 kDa 66 kDa 42 kDa 10 m k b f i Co ntr ol 23 1 VA MP 3-K D- g1 VA MP 3-K D- g2 0 20 40 60 80 Su rfa ce C D 73 + ce lls (% ) p < 0.0001 p < 0.0001 Co ntr ol 46 8 VA MP 3-K D- g1 VA MP 3-K D- g2 0 20 40 60 80 Su rfa ce C D 73 + ce lls (% ) p < 0.0001 p < 0.0001 MD A- MB 46 8 + 1 g/m l T HB S1 0.0 0.5 1.0 1.5 2.0 Ad en os in e co nc en tra tio n ( M ) p = 0.0007 MD A- MB 23 1 + 1 g/m l T HB S1 0 1 2 3 4 Ad en os in e co nc en tra tio n ( M ) p = 0.0009 CD73 CD73 MGAT1 Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly VAMP3 G ly Gly G ly G ly G ly G ly G ly G ly Gly G ly G ly G ly Gly T cells AMP Adenosine X Transport vesicles VAMP3 Failure on CD73 membrane translocationMGAT1 Nature Communications | (2025) 16:3552 10 An Plasmid Preparation:Article Title: MGAT1-Guided complex N-Glycans on CD73 regulate immune evasion in triple-negative breast cancer. Article Snippet: We initially developed an in vitro high-throughput screening assay to monitor the effect on MGAT1 enzymatic activity of a library of putative inhibitors (Fig. 6a). dc e g h j l m N401 N56 N311 N333 Open (inactive) CD73 dimer Closed (active) CD73 dimer Actin MGAT1-OE MGAT1-KD CD73 Dimer CD73 Monomer MDA-MD468 WT Glutaraldehyde - + - + - + 150 kDa 75 kDa 50 kDa MDA-MB468 MGAT1-OE MGAT1-KD SSC-B-H M em br an e C D 73 14.8% 27.3% 2.69% 5 mCD73 Membrane Dye MDA-MB468 MGAT1-OE MGAT1-KD 10 m a CD73 Split-GFP Scheme GFP 1-10 Linker 25aa WT/4NQ/480-537 truncation CD73 GFP 11x7 Linker 25aa WT/4NQ/480-537 truncation CD73 WT CD73 4NQ-CD73 Dimer-deficient CD73 40 x W at er Z oo m 2 .3 CD73 Dimer 00.100.1- 0.00 AD A TG FB 3 C XC L1 4 TG FB R 3 TG FB 2 TG FB R 1 C XC L1 2 TG FB 1 TH BS 3 C XC L9 TH BS 4 C XC L1 6 C XC L1 0 AD A2 EN TP D 1 N T5 E TH BS 2 TH BS 1 M G AT 1 id MGAT1 THBS1 THBS2 NT5E ENTPD1 ADA2 CXCL10 CXCL16 THBS4 CXCL9 THBS3 TGFB1 CXCL12 TGFBR1 TGFB2 TGFBR3 CXCL14 TGFB3 ADA id MDA-MB231 MGAT1 CD73 Actin WT 468 Control vector MGAT1 -OE MGAT1 -OE MGAT1 -KD1 MGAT1 -KD2 75 kDa 50 kDa MDA-MB468 MGAT1 CD73 Actin WT 468 Control vector MGAT1 -OE MGAT1 -OE MGAT1 -KD1 MGAT1 -KD2 75 kDa 50 kDa 50 kDa 42 kDa 50 kDa 42 kDa MDA-MB468 CD73 Actin Co ntr ol En do H PN Ga se F WT Co ntr ol En do H PN Ga se F MGAT1-OE Co ntr ol En do H PN Ga se F MGAT1-KD 75 kDa 50 kDa 42 kDa 42 kDa Actin MGAT1 -OE MGAT1 -KD CD73 Dimer CD73 Monomer MDA-MD468 WT 146 kDa 66 kDa 42 kDa 10 m k b f i Co ntr ol 23 1 VA MP 3-K D- g1 VA MP 3-K D- g2 0 20 40 60 80 Su rfa ce C D 73 + ce lls (% ) p < 0.0001 p < 0.0001 Co ntr ol 46 8 VA MP 3-K D- g1 VA MP 3-K D- g2 0 20 40 60 80 Su rfa ce C D 73 + ce lls (% ) p < 0.0001 p < 0.0001 MD A- MB 46 8 + 1 g/m l T HB S1 0.0 0.5 1.0 1.5 2.0 Ad en os in e co nc en tra tio n ( M ) p = 0.0007 MD A- MB 23 1 + 1 g/m l T HB S1 0 1 2 3 4 Ad en os in e co nc en tra tio n ( M ) p = 0.0009 CD73 CD73 MGAT1 Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly VAMP3 G ly Gly G ly G ly G ly G ly G ly G ly Gly G ly G ly G ly Gly T cells AMP Adenosine X Transport vesicles VAMP3 Failure on CD73 membrane translocationMGAT1 Nature Communications | (2025) 16:3552 10 An Blocking Assay:Article Title: MGAT1-Guided complex N-Glycans on CD73 regulate immune evasion in triple-negative breast cancer. Article Snippet: We initially developed an in vitro high-throughput screening assay to monitor the effect on MGAT1 enzymatic activity of a library of putative inhibitors (Fig. 6a). dc e g h j l m N401 N56 N311 N333 Open (inactive) CD73 dimer Closed (active) CD73 dimer Actin MGAT1-OE MGAT1-KD CD73 Dimer CD73 Monomer MDA-MD468 WT Glutaraldehyde - + - + - + 150 kDa 75 kDa 50 kDa MDA-MB468 MGAT1-OE MGAT1-KD SSC-B-H M em br an e C D 73 14.8% 27.3% 2.69% 5 mCD73 Membrane Dye MDA-MB468 MGAT1-OE MGAT1-KD 10 m a CD73 Split-GFP Scheme GFP 1-10 Linker 25aa WT/4NQ/480-537 truncation CD73 GFP 11x7 Linker 25aa WT/4NQ/480-537 truncation CD73 WT CD73 4NQ-CD73 Dimer-deficient CD73 40 x W at er Z oo m 2 .3 CD73 Dimer 00.100.1- 0.00 AD A TG FB 3 C XC L1 4 TG FB R 3 TG FB 2 TG FB R 1 C XC L1 2 TG FB 1 TH BS 3 C XC L9 TH BS 4 C XC L1 6 C XC L1 0 AD A2 EN TP D 1 N T5 E TH BS 2 TH BS 1 M G AT 1 id MGAT1 THBS1 THBS2 NT5E ENTPD1 ADA2 CXCL10 CXCL16 THBS4 CXCL9 THBS3 TGFB1 CXCL12 TGFBR1 TGFB2 TGFBR3 CXCL14 TGFB3 ADA id MDA-MB231 MGAT1 CD73 Actin WT 468 Control vector MGAT1 -OE MGAT1 -OE MGAT1 -KD1 MGAT1 -KD2 75 kDa 50 kDa MDA-MB468 MGAT1 CD73 Actin WT 468 Control vector MGAT1 -OE MGAT1 -OE MGAT1 -KD1 MGAT1 -KD2 75 kDa 50 kDa 50 kDa 42 kDa 50 kDa 42 kDa MDA-MB468 CD73 Actin Co ntr ol En do H PN Ga se F WT Co ntr ol En do H PN Ga se F MGAT1-OE Co ntr ol En do H PN Ga se F MGAT1-KD 75 kDa 50 kDa 42 kDa 42 kDa Actin MGAT1 -OE MGAT1 -KD CD73 Dimer CD73 Monomer MDA-MD468 WT 146 kDa 66 kDa 42 kDa 10 m k b f i Co ntr ol 23 1 VA MP 3-K D- g1 VA MP 3-K D- g2 0 20 40 60 80 Su rfa ce C D 73 + ce lls (% ) p < 0.0001 p < 0.0001 Co ntr ol 46 8 VA MP 3-K D- g1 VA MP 3-K D- g2 0 20 40 60 80 Su rfa ce C D 73 + ce lls (% ) p < 0.0001 p < 0.0001 MD A- MB 46 8 + 1 g/m l T HB S1 0.0 0.5 1.0 1.5 2.0 Ad en os in e co nc en tra tio n ( M ) p = 0.0007 MD A- MB 23 1 + 1 g/m l T HB S1 0 1 2 3 4 Ad en os in e co nc en tra tio n ( M ) p = 0.0009 CD73 CD73 MGAT1 Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly VAMP3 G ly Gly G ly G ly G ly G ly G ly G ly Gly G ly G ly G ly Gly T cells AMP Adenosine X Transport vesicles VAMP3 Failure on CD73 membrane translocationMGAT1 Nature Communications | (2025) 16:3552 10 An |